Insulin Resistance Protocol

Insulin resistance happens when your body’s cells stop responding well to insulin — the hormone that helps move sugar from your blood into your cells for energy. As a result, your body pumps out more insulin just to keep blood sugar normal.

If this cycle continues, it can lead to type 2 diabetes and other issues.

Common signs and symptoms:

• High blood sugar (consider HbA1c) or insulin (consider c-peptide) on labs

• Crashing after meals, fatigue, brain fog

• Weight gain around the waist

• Increased hunger and cravings

• High blood pressure

• High triglycerides and low HDL 

• Hormone imbalances (e.g., PCOS, testosterone changes)

• Dark skin patches around the neck, armpits, or groin

Why Does It Happen? (Root Causes)

Insulin resistance is not just about eating too much sugar or sitting too much. Those things contribute, but they are not the root of the problem. At its core, insulin resistance is a cellular communication breakdown. Your cells stop responding to insulin because something is interfering with how they receive and act on its signal. Three main mechanisms drive this breakdown:

1. Cell Membrane Health and Fluidity

Every cell is wrapped in a membrane made up of phospholipids, cholesterol, and proteins. This thin but powerful barrier is more than just a wall. It is a dynamic communication hub that controls what enters and exits the cell and allows signals like insulin to dock and activate key processes inside.

For insulin to do its job, transport proteins called GLUT4 (glucose transporters) must travel to the cell surface and embed themselves into the membrane so they can pull glucose inside. If the membrane’s structure is altered, GLUT4 cannot anchor properly or function efficiently, which is one of the earliest steps toward insulin resistance.

Several key factors determine how well your cell membranes work:

1.1 Temperature and Fluidity

The flexibility of the membrane depends heavily on temperature.

• Low temperatures make the membrane more rigid. Phospholipids pack tightly together, slowing the movement of molecules across the membrane and reducing how well insulin signals are received.

• High temperatures increase membrane fluidity, but too much fluidity can make the membrane unstable and overly permeable. Extreme heat can also denature membrane proteins such as GLUT4 or insulin receptors, disrupting their function.

This is why body temperature is one of the most important metabolic signals. It reflects how much energy your cells are producing. If thyroid function is low, metabolic rate slows down and less internal heat is generated, which causes membranes to stiffen and signaling to weaken. Similarly, in states of anemia or low iron, oxygen delivery and mitochondrial activity decline, leading to less heat and poorer membrane responsiveness. Healthy metabolic function requires membranes that are flexible but stable. They must be fluid enough for transport proteins to move and embed, yet structured enough to protect the cell and maintain balance.

1.2 pH and Charge Distribution

The cell membrane is sensitive to changes in pH (the acidity or alkalinity of the environment). Shifts in pH can alter the charge distribution on membrane proteins and phospholipids. This change affects how insulin receptors and transport proteins behave and how well the cell responds to insulin. Chronic acidosis (too much acidity) or alkalosis (too much alkalinity) can reduce membrane responsiveness and make it harder for glucose to enter.

1.3 Cholesterol and Lipid Composition

Cholesterol is a critical structural component that regulates membrane fluidity.

• Too much cholesterol makes the membrane too stiff, slowing transport and reducing insulin sensitivity.

• Too little cholesterol makes the membrane too loose, destabilizing protein function and allowing excessive permeability.

The types of fats you consume also matter. Diets high in oxidized seed oils or trans fats can integrate into the membrane and disrupt its structure. On the other hand, omega-3 fatty acids from foods such as salmon, sardines, and flax improve membrane flexibility and support insulin receptor function.

1.4 Proteins and Transport Capacity

Membrane proteins, including receptors, enzymes, and transport channels, are essential for cell signaling and nutrient transport. Their presence and structure determine how well insulin signals are received and how efficiently glucose moves into the cell. Damage from oxidative stress, inflammation, or toxins can change the shape of these proteins and reduce their effectiveness.

1.5 Toxins and Membrane Integrity

Toxins are one of the most common disruptors of membrane structure. Alcohol, heavy metals, certain chemicals, and persistent environmental pollutants can dissolve parts of the lipid bilayer or oxidize the fats that make up the membrane. This weakens its barrier function and alters the activity of insulin receptors and transport proteins. Over time, toxin buildup contributes to chronic inflammation and metabolic dysfunction, which worsens insulin resistance.

2. Electrical Charge and EZ Water (The Cell’s “Battery”)

Cells are not just bags of water. They are electrical systems. The inside and outside of a cell have different charges, creating a membrane potential that helps signals like insulin work properly. Inside the cell, water is not just water. It forms structured layers called Exclusion Zone (EZ) water, which carry charge and support biochemical reactions.

If a cell loses its charge (often from chronic stress, dehydration, nn-EMF exposure, or mineral imbalances) or has too little EZ water, it struggles to bring GLUT4 to the surface. Even if insulin is present, the "signal" may never reach the membrane.

What you can do:

• Support charge creation: Daily sunlight exposure, grounding (barefoot contact with the earth), and hydration with mineral-rich water all help cells build and maintain charge.

• Optimize key minerals: Potassium and magnesium are crucial for maintaining membrane potential. Most people need more potassium (about 5000 mg per day from food) and sufficient magnesium.

• Avoid constant EMF stress: Reduce unnecessary device exposure and prioritize deep sleep to restore cellular charge.

3. Mitochondrial Energy and Signaling

Even if the membrane is healthy and charged, your cells still need energy (ATP) to move GLUT4 transporters into place and process incoming glucose. The "engines" that make this energy are your mitochondria, and when they are dysfunctional, insulin signaling slows down dramatically.

Low mitochondrial function can result from poor nutrition, chronic inflammation, toxin exposure, or circadian disruption. Without enough ATP, the cell essentially "ignores" insulin because it cannot afford to take on more fuel.

What you can do:

• Prioritize circadian rhythm: Mitochondria are highly time-sensitive. Morning sunlight, regular sleep, and meal timing all help optimize their function.

• Feed your mitochondria: Nutrients like B vitamins, CoQ10, and minerals (especially magnesium, copper, and iron) are essential for ATP production.

• Reduce toxin load: Excessive alcohol, heavy metals, and mold toxins can damage mitochondria. Support drainage pathways and minimize exposure.

Secondary Drivers (Lifestyle and Environmental Factors)

These lifestyle factors worsen the three root causes above. They are not the origin of the problem, but they accelerate it:

• Poor diet quality: Refined carbs, processed oils, and low-quality fats damage membranes and flood cells with fuel they cannot process.

• Low protein and muscle mass: Muscle is the body's main "glucose sink." Less muscle means lower insulin sensitivity.

• Sedentary lifestyle: Movement helps shuttle GLUT4 to the membrane. Lack of exercise makes cells more resistant.

• Chronic snacking: Frequent eating keeps insulin high, exhausting receptor function.

• Chronic stress: Elevated cortisol raises blood sugar and disrupts membrane charge.

• Poor sleep and circadian rhythm: Irregular sleep blunts mitochondrial energy production.

• Nutrient deficiencies: Low magnesium, chromium, potassium, and omega-6/omega-3s impair every step of insulin signaling.

Dietary Considerations

Nutrition has a powerful influence on insulin sensitivity because it directly affects how much insulin your body needs to release, how your cell membranes are built, how much energy your mitochondria can produce, and whether glucose can actually enter your cells. Both excessive carbohydrates and excessive fats can drive insulin resistance, but they do so in very different ways.

Takeways:

• Carb resistance happens from too much sugar + not enough movement.

• Fat resistance happens from too many bad fats + extra weight.

• High-carb diets can work for active, lean people who eat whole, seasonal carbs.

• High-fat/keto diets can help overweight or diabetic people by lowering blood sugar and insulin needs — but only if the fats are mostly healthy ones.

How to Assess It

Work with your labs and track progress. The most reliable markers are:

• C-peptide (best marker for insulin production)

• Fasting insulin

• HbA1c (3-month average of blood sugar regulation)

• Fasting glucose

• Triglycerides + HDL cholesterol

(See Advanced Functional Tests for detailed interpretation.)

Order these tests on Ulta Lab:

• C-peptide
• Fasting insulin, fasting glucose, HbA1c
• Lipid panel

Lifestyle Reset Steps

Reversing insulin resistance is not about willpower or cutting calories. It is about restoring how your cells function so they can respond to insulin again. Every lifestyle choice you make either builds or breaks down the three foundations of insulin sensitivity: membrane structure, cellular charge, and mitochondrial energy. The steps below are designed to support all three.

1. Regulate Your Circadian Rhythm (Most Important Step)

Your circadian rhythm is the master clock that governs every metabolic process, including insulin release, GLUT4 activation, mitochondrial energy production, and hormonal balance. When your internal timing is disrupted, even the best diet will not fully restore insulin sensitivity.

Morning light exposure:

• See the sunrise daily for at least 10 minutes. This simple habit sets your body’s internal clock and initiates hormonal signaling that supports insulin balance.

• Exposing your skin to early morning light also helps build structured water (EZ water) within cells. Red and infrared wavelengths from natural light are especially effective at supporting this process.

Midday light and movement:

• Spend time outdoors during the day, particularly when UVA and UVB light are present. These wavelengths help your cells generate more structured water, support mitochondrial function, and naturally optimize vitamin D production.

• No supplement can fully replace the benefits of real sunlight for metabolic and hormonal health.

Evening light hygiene:

• Block artificial blue light after sunset by using red-lens glasses such as Viva Rays or RA Optics. This supports the release of melatonin, the hormone that signals the start of cellular repair and helps regulate blood sugar overnight.

• Try to finish your last meal near sunset. Eating late at night keeps insulin elevated and disrupts circadian-driven metabolic processes.

Sleep consistency:

• Go to bed and wake up at consistent times. This stabilizes insulin and cortisol rhythms and allows mitochondria to produce energy more efficiently during the day.

A well-regulated circadian rhythm improves membrane fluidity, enhances GLUT4 signaling, and supports the production of ATP (cellular energy), all of which are essential for restoring insulin sensitivity at the root level.

2. Nutrition and Eating Patterns

Your diet does more than fuel your body. It builds the physical structure of your cells, determines how much insulin you produce, and shapes how effectively your mitochondria generate energy. The right approach nourishes the system without overwhelming it.

• Start your day with a protein-rich meal (30–40 g) timed with the first rise of UVA light. This stabilizes blood sugar, signals muscle to respond to insulin, and supports mitochondrial function. Use the Circadian app to check UVA timing in your location, as it changes seasonally.

• Structure meals with no snacking. Eat every 3–4 hours and follow this order to minimize glucose spikes and improve nutrient absorption:

  1. Vegetables

  2. Protein and fat

  3. Grains and starches (bread, pasta, rice)

  4. Dessert (keep minimal while reversing insulin resistance)

• Implement an overnight fast of 12–16 hours. Women should shorten fasts to 12–14 hours during the luteal phase to support hormone balance.

• Match macronutrients to the season:

  • Winter: 40% carbs / 30% fat / 30% protein or 20% carbs / 60% fat / 20% protein

  • Other seasons: Greater flexibility if you are active and aligned with your circadian rhythm

• Avoid: Seed oils (canola, corn, cottonseed, grapeseed, peanut, rice bran, safflower, soybean), fried foods, and alcohol. These damage membranes, increase oxidative stress, and impair GLUT4 transport.
• Cook with: Butter, ghee, or tallow for stability. Use olive oil for cold dishes or seasoning.

Support digestion and insulin sensitivity:

• Add 1 tbsp of apple cider vinegar in water before meals (use a straw to protect tooth enamel).

• Mineralize water by adding a pinch of natural salt to each cup to support membrane potential and structured water formation.

Eating this way reduces insulin demand, supports healthy membrane composition, and helps build the charge your cells need to respond properly to insulin.

3. Exercise and Movement

Muscle tissue is the most insulin-sensitive tissue in your body. Movement helps GLUT4 transporters move to the cell membrane and pull glucose inside, while also building mitochondria and improving circulation.

• After meals: Take a 15–20 minute walk or do 20–40 bodyweight squats to quickly lower blood sugar and enhance insulin signaling.

• Strength training: Lift weights at least 3 times per week to increase muscle mass, which acts as a glucose sponge and improves metabolic flexibility.

• For PCOS or weight loss support: Combine strength training with cardio 3 times per week to further improve insulin sensitivity and hormone balance.

Consistent movement improves charge, builds mitochondrial capacity, and enhances GLUT4 responsiveness, reversing the core dysfunctions behind insulin resistance.

4. Key Nutrients

Nutrients are not just “fuel.” They are the raw materials for building membranes, maintaining charge, and generating energy. Without them, the other steps cannot fully work.

Track with Cronometer and aim for:

• Protein: At least 1.2 g per kg of body weight per day (this is the minimum).

• Potassium: Around 5000 mg per day (more important than Cronometer’s default target).

• Sodium: About 4500 mg per day if potassium is optimal.

• Omega-3s: 3–4 servings of fatty fish per week (such as sardines, salmon, or mackerel).

• Omega-6s: 1 serving per day of seeds such as sunflower, pumpkin, or flax.

These nutrients support membrane structure, maintain electrical gradients, and fuel ATP production, which together restore insulin sensitivity.

5. Retesting and Tracking Progress

Reversal of insulin resistance is measurable, and tracking allows you to see progress even before symptoms fully resolve.

• Re-test C-peptide and fasting insulin every 1–3 months to assess insulin production and sensitivity.

• Re-test HbA1c every 3 months to monitor long-term glucose control.

• Track non-lab markers such as waist circumference, energy levels, cravings, and sleep quality, which often improve before lab values change.

5. Targeted Supplements (Optional but Powerful Support)

Lifestyle changes are the foundation of reversing insulin resistance, but certain supplements can significantly accelerate progress by addressing the three key cellular issues: membrane function, cellular charge, and mitochondrial energy. These should always be introduced in a strategic order, starting with the most foundational supports.

1. BodyBio PC – 1 tsp daily with meals
Phosphatidylcholine (PC) is one of the most critical building blocks of cell membranes. Healthy, flexible membranes allow insulin receptors and GLUT4 transporters to function properly and improve how glucose enters the cell. Supporting membrane structure is often the first and most impactful step in restoring insulin sensitivity.

2. TUDCA – 1000–1750 mg/day in divided doses on an empty stomach
TUDCA supports liver and bile flow, which is essential for fat digestion, detoxification, and healthy metabolic signaling. A well-functioning liver improves how the body handles glucose and insulin, and it helps reduce the toxic load that can damage membranes and mitochondria. Addressing liver stress is a key part of rebuilding proper insulin response.

If more support is needed after addressing the above:

3. Berberine (Thorne) – 1-3 capsules per day, with meals
Berberine activates AMPK, a metabolic “master switch” that improves GLUT4 translocation, increases glucose uptake, and enhances mitochondrial energy production. It also helps reduce post-meal blood sugar spikes and improves lipid metabolism.

4. CellCore GCO – 1-2 capsules per day, with or without meals
GCO provides a targeted blend of botanicals that support glucose regulation, insulin receptor sensitivity, and detoxification pathways. It is best used when lifestyle changes and foundational supplements alone are not sufficient.

✅ Key Takeaway for Members

Insulin resistance is reversible, but only if you address the deeper cellular dysfunctions behind it. The fastest results come from:

• Aligning your lifestyle with your circadian rhythm to support mitochondrial energy and charge

• Eating real, seasonal food that builds healthy membranes and minimizes insulin demand

• Moving after meals and building muscle to increase GLUT4 activity

• Avoiding seed oils, alcohol, and constant snacking to prevent membrane damage

• Supporting with key nutrients and targeted supplements if needed

 💬 Community Discussion Only

Comments here are meant for sharing feedback, insights, or experiences with other members. Paola does not respond to questions here, but she reads everything and may occasionally pop in to share additional knowledge or refine protocols based on community feedback.

📍 For direct questions or support with applying this protocol, please post inside the Inner Circle Q&A instead.